Human protease-activated receptor 2 (PAR2) is one of 4 members in a unique subclass of the Class A G protein- coupled receptor superfamily. Receptor activation involves the proteolytic uncovering of a cryptic N-terminus receptor sequence that forms a tethered ligand and binds to the receptors within the extracellular loop 2. This activates the receptors to initiate multiple signaling cascades. In addition, short peptides (5-6 amino acids) synthesized based on the proteolytically revealed motif can activate the receptors without the cleavage by enzymes. PAR2 is activated by trypsin and trypsin-like enzymes, and the cleavage site has been identified at residue Arg 36. PAR2 plays an important role in the regulation of cardiovascular, gastrointestinal, and skin function. PAR2 is expressed in human vascular tissues, pancreas, kidney, colon, liver, small intestine, and some tumor cell lines (1, 2).
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: Use 10ul to label 1x10e5 cells or 50ul of packed whole blood.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C. Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
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