Human p21-GTPase-activated protein kinase 1 (PAK1) is a functional homolog of STE20 of Saccharomyces cerevisiae. PAK1 is a downstream effector of the Rho-family small GTPases Rac and Cdc42. A serine/threonine kinase, PAK1 induces the formation of filopodia and lamellipodia in Swiss 3T3 cells. PAK1 inhibits myosin light chain kinase, and appears to play a role in the regulation of apoptosis by phosphorylating the death agonist Bad. Membrane targeting of PAK1 induces neurite outgrowth. PAK1 may also regulate NFkB activation.
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.