Home  >  Products  >  p53, phosphorylated (Ser392) (Antigen NY CO 13, Antigen NY-CO-13, Cellular Tumor Antigen p53, Cys 51 Stop, HGNC:11998, LFS1, p53 Cellular Tumor Antigen, p53 Tumor Suppressor, Phosphoprotein p53, Transformation Related Protein 53, TRP53, Tumor Protein p53,

p53, phosphorylated (Ser392) (Antigen NY CO 13, Antigen NY-CO-13, Cellular Tumor Antigen p53, Cys 51 Stop, HGNC:11998, LFS1, p53 Cellular Tumor Antigen, p53 Tumor Suppressor, Phosphoprotein p53, Transformation Related Protein 53, TRP53, Tumor Protein p53,

Cat no: P1001-27U


Supplier: United States Biological
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p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. p53 can apparently be phosphorylated by ATM, ATR, and DNAPK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage. Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability and activity. p53 is phosphorylated at Ser392 in vivo and by CAK in vitro. Phosphorylation of p53 at Ser392 is altered in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53. p53 is phosphorylated at Ser6 and Ser9 by ck1d and ck1e both in vitro and in vivo. Phosphorylation of p53 at Ser46 is important in regulating the ability of p53 to induce apoptosis. In vivo phosphorylation of p53 at Ser33 by cdk7/cyclin H and in response to UV irradiation has been observed. Applications: Suitable for use in Western Blot and Immunohistochemistry (paraffin-embedded sections). Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: P1001-27U
Reactivities: Human, Mouse
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2, 50% glycerol. No preservative added.
P type: Mab
Isotype: IgG1
Purity: Purified
References: Kohn KW Molecular interaction map of the mammalian cell cycle control and DNA repair systems. Mol Biol Cell 10:2703-34 (1999). PubMed: 10436023 Lu H et al. The CDK7-cycH-p36 complex of transcription factor IIH phosphorylates p53, enhancing its sequence-specific DNA binding activity in vitro. Mol Cell Biol 17:5923-34 (1997). PubMed: 9315650 Hao M et al. Mutation of phosphoserine 389 affects p53 function in vivo. J Biol Chem 271:29380-5 (1996). PubMed: 8910602 Lohrum M & Scheidtmann KH Differential effects of phosphorylation of rat p53 on transactivation of promoters derived from different p53 responsive genes. Oncogene 13:2527-39 (1996). PubMed: 9000127
Additional info: This antibody detects a 53kD protein, corresponding to the apparent molecular mass of phosphorylated p53, on Western blots in samples of human origin. It does not detect dephosphorylated p53. Species Crossreactivity: Reacts with Human and Mouse. Predicted to react with Rabbit (85% identity with immunogen), canine (85% identity with immunogen), Guinea pig (85% identity with immunogen), Monkey (92% identity with immunogen) and Pig (85% identity with immunogen) due to sequence homology.

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