Due to the various splice variants, the trk proteins exist as variably glycosylated entities with the major forms having molecular weights of 140-145kD, 110kD and the unglycosylated form of 80kD. trkB contains 33.3% carbohydrate by weight representing modification on 10 of 12 N-glycosylation sites. The primary ligand for trkB is BDNF which induces the phosphorylation of the protein and subsequent binding of PLC-gamma via SH2 domains. TrkB may function to modulate neuronal responses to the neurotrophins acting through trkB such as BDNF.
Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Immunofluorescence: 5-10ug/ml
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.