NQO1 (Quinone 1) is a flavoenzyme that catalyzes two-electron reductive metabolism and
detoxification of quinones and their derivatives leading to protection of cells against redox cycling and oxidative stress (1).NQO1 is mainly cytosolic, but its nuclear presence suggests its involvement in both chemoprevention and bioactivation of DNA-damaging antitumor agents (2).NQ01 may also play a role in the bioactivation of antitumor quinones such as mitomycin C (MMC), and has been proposed to have a protective effect against cancer caused by quinones and their metabolic precursors (3-4).
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Western Blot: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.