Nestin is a type IV intermediate filament protein expressed by neuroepithelial stem cells and which has been proposed to represent a marker for putative islet stem cells. Nestins are expressed by several types of cells during development, mostly in dividing cells of the Central Nervous System, Peripheral Nervous System and myogenic tissues. It also represents the progenitor population of neural stem cell origin. The role of nestin in dynamic cells, particularly structural organization of the cell, appears strictly regulated by phosphorylation, especially its integration into heterogeneous intermediate filaments together with vimentin or a-internexin. Nestin has recently received attention as a marker for detecting newly formed endothelial cells.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 3-5ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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