Home  >  Products  >  Neomycin Phosphotransferase II (NPT II, NPT-II, Aminoglycoside 3' Phosphotransferase, APH(3')II, APH(3')-II, aphA, EC 2.7.1.95, Kan, Kanamycin Kinase Type II, Kanamycin Kinase Type VIII, Kanamycin Resistance Gene, Neo, Neomycin Kanamycin Phosphotransferas
Neomycin Phosphotransferase II (NPT II, NPT-II, Aminoglycoside 3' Phosphotransferase, APH(3')II, APH(3')-II, aphA, EC 2.7.1.95, Kan, Kanamycin Kinase Type II, Kanamycin Kinase Type VIII, Kanamycin Resistance Gene, Neo, Neomycin Kanamycin Phosphotransferas

Neomycin Phosphotransferase II (NPT II, NPT-II, Aminoglycoside 3' Phosphotransferase, APH(3')II, APH(3')-II, aphA, EC 2.7.1.95, Kan, Kanamycin Kinase Type II, Kanamycin Kinase Type VIII, Kanamycin Resistance Gene, Neo, Neomycin Kanamycin Phosphotransferas

Cat no: N2008-05C

Supplier: United States Biological
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The neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: N2008-05C
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Western Blot
Size: 100ul
Form: Supplied as a liquid, 0.03% sodium azide.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein A affinity chromatography.
References: 1. Landis WG et.al Human and ecological Risk Assessment 6(5): 875-899. 2. Stewart CN et.al BioTechniques 29(4): 832-843. 3. Thomson JA et.al Journal of Food Science 66(2): 188-193.
Additional info: Recognizes human Neomycin Phosphotransferase II.

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