Home  >  Products  >  Nedd8 (Neural Precursor Cell Expressed Developmentally Down-regulated 8, NEDD-8, MGC104393, MGC125896, MGC125897, NED8, Neddylin, Neural Precursor Cell Expressed Developmentally Down-regulated Gene 8, Rub1, Ubiquitin-like Protein Nedd8, Ubiquitin-like Pro

Nedd8 (Neural Precursor Cell Expressed Developmentally Down-regulated 8, NEDD-8, MGC104393, MGC125896, MGC125897, NED8, Neddylin, Neural Precursor Cell Expressed Developmentally Down-regulated Gene 8, Rub1, Ubiquitin-like Protein Nedd8, Ubiquitin-like Pro

Cat no: N0580-02D


Supplier: United States Biological
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NEDD8 (Neural precursor cell-Expressed Developmentally Down-regulated genes) is an ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis (1). NEDD8 has been shown to conjugate to nuclear proteins in a manner analogous to ubiquination and sentrinization. The conjugation process is though to be catalyzed by thee enzymes; E1 (NEDD8-activation), E2 (NEDD8-conjugation) and E3 (NEDD8-ligation). NEDD8 is known to conjugate to Cul-4A and other cullins proteins via the carboxy-terminal Gly residue for ubiquitinylation (3) while NUB1 will negatively regulate its expression (4). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Immunohistochemistry: 1:100-1:250 Immunocytochemistry: 1:100-1:250 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: N0580-02D
Reactivities: Human
Hosts: Rabbit
Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Kumar, S., et al. 1993. Biochem. Biophys. Res. Commun. 195: 393-399 2. Gong, L., et al. (1999) J. Biol. Chem. 274, 12036-12042 3. Kipreos, E.T., et al. 1996. Cell 85: 829-839 4. Katsumi Kito,et al. Biol. Chem., Vol. 276, Issue 23, 20603- 20609, June 8, 2001
Additional info: Recognizes human NEDD8 bound to cullins proteins (neddylated cullins) as well as free NEDD8.

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