Ubiquitin-like proteins are an expanding family of proteins that includes UCRP, sentrin1/SUMO1, sentrin2, sentrin3, Rub1, and Nedd8. Nedd8 is a small protein (9kD) that shows the highest similarity to the ubiquitin protein (60% amino acid sequence identity). It was identified as one of multiple neural precursor cell-expressed developmentally down-regulated genes in mice.1 Nedd8 mRNA is highly restricted to the heart and skeletal muscle in adult human tissues, and is down regulated in mouse embryos.
Suitable for use in ELISA and Western Blot. Other applications not tested.
Western Blot: 1:100-1:2000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.