Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Cardiac MHC exists as two isoforms in humans, alpha-cardiac MHC and beta-cardiac MHC. These two isoforms are expressed in different amounts in the human heart. During normal physiology, beta-cardiac MHC is the predominant form, with the alpha-isoform contributing around only 7% of the total MHC. Mutations of the MHC genes are associated with several different dilated and hypertrophic cardiomyopathies.
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Immunohistochemistry (Frozen): 10ug/ml tissue treatment may reduce or destroy the myosin binding sites
Western Blot: 1:100-1:2000. Detects a band at ~223kD
Immunocytochemistry (IF): 1:10-1:2000
Indirect Immunofluorescence (Frozen): Binds to A-band of thick filament of cardiac and skeletal muscle myosin and indirect immunoperoxidase staining of Carnoy fixed tissues.
Optimal dilutions to be determined by the researcher.
Human ventricular myosin heavy chains: 3.33 x10e8/M Human atrial myosin heavy chains: 1.48 x10e8/M
Human skeletal muscle myosin: 1.06 x10e8/M
Canine cardiac myosin heavy chains: 2.06 x10e8/M
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.