Home  >  Products  >  Myosin, Cardiac, Heavy Chain (Alpha MHC, ASD3, CMD1S, CMH1, MGC138376, MGC138378, MPD1, MYH6, MYH7, MYHC, MyHC-alpha, MYHCA, MyHC-beta, MYHCB, Myosin Heavy Chain Cardiac Muscle alpha Isoform, Myosin Heavy Chain Cardiac Muscle beta Isoform, Myosin Heavy Po

Myosin, Cardiac, Heavy Chain (Alpha MHC, ASD3, CMD1S, CMH1, MGC138376, MGC138378, MPD1, MYH6, MYH7, MYHC, MyHC-alpha, MYHCA, MyHC-beta, MYHCB, Myosin Heavy Chain Cardiac Muscle alpha Isoform, Myosin Heavy Chain Cardiac Muscle beta Isoform, Myosin Heavy Po

Cat no: M9850-11C


Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Cardiac MHC exists as two isoforms in humans, alpha-cardiac MHC and beta-cardiac MHC. These two isoforms are expressed in different amounts in the human heart. During normal physiology, beta-cardiac MHC is the predominant form, with the alpha-isoform contributing around only 7% of the total MHC. Mutations of the MHC genes are associated with several different dilated and hypertrophic cardiomyopathies. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:100-1:2000 Immunohistochemistry: 1:10-1:2000 Immunohistochemistry (Frozen): 10ug/ml tissue treatment may reduce or destroy the myosin binding sites Immunohistochemistry(Paraffin): 1:400 Western Blot: 1:100-1:2000. Detects a band at ~223kD Immunocytochemistry (IF): 1:10-1:2000 Indirect Immunofluorescence (Frozen): Binds to A-band of thick filament of cardiac and skeletal muscle myosin and indirect immunoperoxidase staining of Carnoy fixed tissues. Optimal dilutions to be determined by the researcher. Affinity Constants: Human ventricular myosin heavy chains: 3.33 x10e8/M Human atrial myosin heavy chains: 1.48 x10e8/M Human skeletal muscle myosin: 1.06 x10e8/M Canine cardiac myosin heavy chains: 2.06 x10e8/M Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: M9850-11C
Reactivities: Human, Mouse, Rat, Bovine, Canine, Porcine, Rabbit
Hosts: Mouse
Applications: ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.4. No preservatives added.
P type: Mab
Isotype: IgG1,k
Purity: Purified
Additional info: Recognizes human myosin alpha and beta heavy chains. Reacts with heavy chains of human myosin isolated from: slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal M5 peptide. (Myosin heavy chain is specific for cardiomyocytes.). It does not react with human platelet, smooth muscle myosin heavy chains, myosin light chains, myosin fragments circulating in blood of patients with myocardial infarction. Species Crossreactivity: bovine, mouse, rabbit, rat, canine and porcine.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*

Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave