Home  >  Products  >  MRP2 (Multidrug Resistance Associated Protein 2, ABC30, ATP-binding Cassette Sub-family C (CFTR/MRP) Member 2, ATP Binding Cassette Sub-family C Member 2, ABCC2, Canalicular Multidrug Resistance Protein, cMRP, Canalicular Multispecific Organic Anion Trans

MRP2 (Multidrug Resistance Associated Protein 2, ABC30, ATP-binding Cassette Sub-family C (CFTR/MRP) Member 2, ATP Binding Cassette Sub-family C Member 2, ABCC2, Canalicular Multidrug Resistance Protein, cMRP, Canalicular Multispecific Organic Anion Trans

Cat no: M4688-20


Supplier: United States Biological
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The two members of the large family of ABC transporters known to confer multidrug resistance in human cancer cells are the MDR1 P-glycoprotein and the multidrug-resistance protein MRP1. MRP1 is an integral membrane protein that contains an MDR-like core, an N-terminal membrane-bound region and a cytoplasmic linker, and it is expressed in various cerebral cells, as well as in lung, testis and peripheral blood. The MRP gene family also includes MRP2, which is alternatively designated cMOAT (for canalicular multispecific organic anion transporter) and MRP3, which are both conjugate export pumps expressed predominantly in hepatocytes. MRP2 localizes exclusively to the apical membrane and is constitutively expressed at a high level in normal liver cells. Conversely, MRP3 localizes to the asolateral membrane where it also mediates the transport of the organic anion S-(2,4-dinitrophenyl-) glutathione toward the basolateral side of the membrane. MRP3 is normally expressed at comparatively lower levels than MRP2 and increases only when secretion across the apical membrane by MRP2 is impaired. MRP6 protein is highly expressed in liver and kidney, whereas MRP4 and MRP5 are detected in various tissues yet at much lower levels of expression. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50%glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: M4688-20
Reactivities: Human
Hosts: Mouse
Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Size: 250ul
Form: As reported
P type: Mab
Isotype: IgG1
Purity: Tissue culture supernatant
References: 1. Versantvoort, C.H., et al. 1995. Regulation by glutathione of drug transport in multidrug-resistant human lung tumour cell lines overexpressing multidrug resistance-associated protein. Br. J. Cancer 72: 82-89. 2. Kool, M., et al. 1997. Analysis of expression of cMOAT (MRP2), MRP3, MRP4 and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines. Cancer Res. 57: 3537-3547. 3. Keppler, D., et al. 1997. Hepatic canalicular membrane 5: expression and localization of the conjugate export pump encoded by the MRP2 (cMRP/cMOAT) gene in liver. FASEB J. 11: 509-516. 4. Bakos, E., et al. 1998. Functional multidrug resistance protein (MRP1) lacking the N-terminal transmembrane domain. J. Biol. Chem. 273:32167-32175. 5. Ortiz, D.F., et al. 1999. MRP3, a new ATP-binding cassette protein localized to the canalicular domain of the hepatocyte. Am. J. Physiol. 276: 1493-1500.
Additional info: Species Crossreactivity: Does not crossreact with MDR1, MRP1, MRP3 or MRP5.

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