MLL is a DNA-binding protein that methylates histone H3 lys4 (H3K4) and positively regulates expression of target genes, including multiple HOX genes. MLL is a frequent target for recurrent translocations in acute leukemias that may be characterized as acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), or mixed lineage (biphenotypic) leukemia (MLL). Leukemogenic MLL translocations encode MLL fusion proteins that have lost H3K4 methyltransferase activity. A key feature of MLL fusion proteins is their ability to efficiently transform hematopoietic cells into leukemia stem cells.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 1:500-1:2000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.