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Midkine (MK), Human BioAssay(TM) ELISA Kit

Midkine (MK), Human BioAssay(TM) ELISA Kit

Cat no: M3896-04

Supplier: United States Biological
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In the Human Midkine ELISA, Calibrators, Quality Controls, and samples are incubated with a rabbit polyclonal anti-human midkine antibody coated in microtiter wells. After two hours of incubation and washing, biotin-labeled polyclonal anti-human midkine antibody is added and incubated with captured midkine for one hour. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour of incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by the addition of acidic solution and the absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of midkine. A standard curve is constructed by plotting the absorbance values against the concentrations of midkine calibrators. The concentrations of unknown samples are determined using this standard curve. Samples: serum, citrate plasma and heparin plasma. Kit Components: M3896-04B: Microtiter Plate, 1x96 wells M3896-04C: Midkine Antibody (Biotin) 1x13ml M3896-04D: Streptavidin (HRP) 1x13ml M3896-04E: Human Standard, 2x1vial M3896-04F: Quality Control 1x1vial M3896-04J: Wash Solution, 10x 1x100ml M3896-04K: Substrate Solution (TMB), 1x13ml M3896-04L: Stop Solution, H2SO4, 1x13ml M3896-04M: Dilution Buffer 1x13ml M3896-04N: Standard Diluent: 1x9ml Storage and Stability: Store all components at 4C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Reconstituted standards and controls can be aliquoted and stored at -20C..
Catalogue number: M3896-04
Reactivities: Human
Applications: ELISA
Size: 1Kit
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