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MHC Class I (H-2 class I histocompatibility antigen, K-B alpha chain precursor, H-2 Class I histocompatibility antigen, K-D alpha chain precursor, H-2 class I histocompatibility antigen, K-K alpha chain precursor, H-2 class I histocompatibility antigen, K

MHC Class I (H-2 class I histocompatibility antigen, K-B alpha chain precursor, H-2 Class I histocompatibility antigen, K-D alpha chain precursor, H-2 class I histocompatibility antigen, K-K alpha chain precursor, H-2 class I histocompatibility antigen, K

Cat no: M3887-49R

Supplier: United States Biological
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The monoclonal antibody specifically reacts with major histocompatibility complex (MHC) class I antigens of the mouse and, therefore, it is a valuable tool for studying cytotoxic T-cell interactions with class I positive antigen presenting cells. The primary immunological function of MHC molecules is to bind and present antigenic peptides on the surfaces of cells for recognition by the antigen-specific T cell receptors (TCRs) of lymphocytes. MHC class I molecules are expressed on the surfaces of most cells and are recognized by CD8-positive cytotoxic T-cells, an essential step for initiating the elimination of virally infected cells by T-cell-mediated lysis. MHC class I molecules are heterodimers composed of an alpha (44kD) and a beta (beta microglobulin, 11kD) subunit. The first two structural domains of the alpha subunit associate to form the peptide-binding pocket. The monoclonal antibody reacts with MHC class I, an antigen that is expressed by all somatic cells at varying levels. Lymphocytes are highly positive, whereas fibroblasts and neurons show only a low level of antigen expression. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 Immunohistochemistry (Frozen): 1:50. The antigen is glutaraldehyde (0.05%), paraformaldehyde (1%) and acetone resistant. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: M3887-49R
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry, Immunohistochemistry
Size: 100ug
Form: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes mouse MHC Class I. Reacts with MHC Class I on the surface of cells of the following haplotypes: H-2Fv, H-2Dd, H-2k,q,s. Weaker reactivity is found in mice of the following haplotypes: H-2p,r,w7,w22. MHC class I molecules of other haplotypes are not recognized. Does not crossreact with human.

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