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MHC Class 2 (beta chain) (FITC)

Cat no: M3887-55C

Supplier: United States Biological
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Human MHC Class II (HLA-DP, DQ & DR) molecules are expressed on dendritic cells, monocytes, macrophages, and myeloid cells. MHC Class II molecules are heterodimers of polymorphic transmembrane a and b chains and generally bind exogenously derived peptides of about 10-17aa MHC Class II molecules interact with the T cell receptor on CD4+ T cells. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 (5ug/ml) labels 5x10e5 human Daudi cells Optimal dilutions to be determined by the researcher. FACS Protocol: 1. Five x10e5 cultured human Daudi cells were pre-incubated with 20ul of human IgG at 300ug/ml (to block non-specific binding), after which they were incubated on ice for 45 minutes with 80ul of M3887-55C at a 1:50 dilution factor (5ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 2.1 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml M3887-55A MHC Class 2 (beta chain). Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: M3887-55C
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Western Blot
Size: 120Tests
Form: Supplied as a liquid in PBS, 5% Glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with FITC.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. T.A. de Kretser, et al,Eur J Immunol 12: 214-221 (1982). 2. R. Busch & J.B. Rothbard J Immunol Methods 134: 1-22. , (1990) 3. K.W. Wacherpfennig & J.L. Strominger, J Exp Med 181:161-168.(1995).
Additional info: Recognizes human monomorphic class II beta chain epitope.

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