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MEK1, phosphorylated (Ser217/221) Sandwich ELISA, BioAssay(TM) Antibody Pair

Cat no: M2865-02E

Supplier: United States Biological
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Phospho-MEK1 (Ser217/221) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our Phospho-MEK1 (Ser217/221) Sandwich ELISA Kit. MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221 (in the activation loop of subdomain VIII) by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The MEK1 Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by Phospho-MEK1/2 (Ser217/221) Detection Antibody and anti-Rabbit IgG, HRP-conjugated antibody. HRP substrate, TMB, is added for color develop- ment. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-MEK1/2 (Ser217/221) protein.
Catalogue number: M2865-02E
Reactivities: Human, Mouse
Size: 1Kit
References: (1) Crews, C.M. et al. (1992) Science 258, 478

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