Home  >  Products  >  MCM7 (Minichromosome Maintenance Protein 7, CDABP0042, CDC47, CDC47 Homolog, DNA Replication Licensing Factor MCM7, Homolog of S. cerevisiae Cdc47, MCM7 Minichromosome Maintenance Deficient 7, Minichromosome Maintainence 7, P1.1-MCM3, P1CDC47, P85MCM, PNA

MCM7 (Minichromosome Maintenance Protein 7, CDABP0042, CDC47, CDC47 Homolog, DNA Replication Licensing Factor MCM7, Homolog of S. cerevisiae Cdc47, MCM7 Minichromosome Maintenance Deficient 7, Minichromosome Maintainence 7, P1.1-MCM3, P1CDC47, P85MCM, PNA

Cat no: M2749-37C


Supplier: United States Biological
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The minichromosome maintenance (MCM7) helicase complex functions to initiate and elongate replication forks. Cell cycle checkpoint signaling pathways regulate DNA replication to maintain genomic stability (1). MCM7 is a putative human homologue of yeast CDC47 and a member of the MCM protein family, which has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Findings indicate that MCM7 protein together with other MCM proteins participates in the regulation of mammalian DNA replication (2). In HeLa cells, depletion of MCM7 with small-interfering RNA suppressed ultraviolet (UV) light- or aphidicolin-induced hChk1 phosphorylation, and abolished UV-induced S-phase checkpoint activation. These results demonstrate that MCM7 plays a direct role in the transmission of DNA damage signals from active replication forks to the S-phase checkpoint machinery in human cells (3). Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:40 Western Blot: 1:10,000 Immunoprecipitation: 1:40 Immunohistochemistry: 1:100-1:250 Immunocytochemistry: 1:100-1:250 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: M2749-37C
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Cortez D, et al. Proc Natl Acad Sci USA 101(27):10078-83 2004 2. Fujita M, et al. J Biol Chem 271(8):4349-54, 1996 3. Tsao CC, et al. EMBO J 23(23):4660-9, 2004
Additional info: Recognizes human MCM7. Species Crossreactivity: Mouse and rat.

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