Human nuclear proteins MCM3 and MCM5 have high sequence similarities with the corresponding yeast proteins known to be required for the initiation of genome replication. At 0.5 M NaCl, the structure-bound nuclear protein can be partially solubilized as a dimer composed of MCM3 and the related protein MCM5. MCM3 forms stable complexes with protein MCM5. These MCM3/MCM5 complexes occur as dimers and in high-molecular-mass complexes (approximately 500kDa). The high-molecular-mass complexes dissociate in 0.5 M NaCl and release MCM3/MCM5 dimers. It has frequently been proposed that the MCM proteins may function as licensing factors for genome replication. Data imply that the active form of an MCM protein is not a monomer, but a protein complex that includes an MCM3/MCM5 dimer (1). Levels of member of the MCM family (MCM2, MCM3, MCM5 and MCM7) gradually increased in a variable manner as KD cells progressed from GO into the G1/S phase. In the GO stage, the amounts of MCM2 and -5 proteins were much lower than those of MCM7 and -3 proteins, suggesting that they are not present in stoichiometric amounts, and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM/P1 complexes (2).
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Flow Cytometry: 1:40
Western Blot: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.