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Marcks, Phosphorylated (Ser152/Ser156) (Myristoylated Alanine-rich C-kinase Substrate, MARCKS, Protein Kinase C Substrate 80kD Protein, Macs)

Marcks, Phosphorylated (Ser152/Ser156) (Myristoylated Alanine-rich C-kinase Substrate, MARCKS, Protein Kinase C Substrate 80kD Protein, Macs)

Cat no: M2375-05M

Supplier: United States Biological
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MARCKS (Myristoylated alanine-rich protein kinase C substrate) is a major PKC substrate that is distributed in various cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic and mitogenesis. PKC phosphorylates Ser152, 156 and 163 of MARCKS, which regulates MARCKS's calcium/calmodulin binding activity and filamentous (F)-actin cross-linking activity. In addition, phosphorylation by PKC results in translocation of MARCKS from membrane to cytoplasm. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: M2375-05M
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% thimerosal, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by affinity chromatography.
Additional info: Recognizes rat MARCKS when phosphorylated at Ser152/156. Species Crossreactivity: mouse and human.

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