MAP kinase-activated protein kinase-2 (MAPKAPK-2), also known as p45 hsp27 kinase, is a 45-54kD serine-threonine protein kinase. MAPKAPK-2 is rapidly phosphorylated and activated in response to stress, cytokines, and chemotactic factors. One of the major substrates of MAPKAPK-2 is hsp27, which stimulates actin polymerization in order to facilitate recovery from destruction of cytoskeleton during cellular stresses. Two isoforms are produced due to alternative splicing of the same gene which differs in their C-terminals. This antibody recognizes both isoforms.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.