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MAPK Family, phosphorylated BioAssay(TM) Sample Kit

Cat no: M2352-41

Supplier: United States Biological
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P44/42 MAPK (Erk1 and Erk2), p38 MAPK and SAPK function in protein kinase cascades that play a critical role in the regulation of cell growth and differentiation and the control of cellular responses to cytokines and stress. p44/42 MAPK (Erk1 and Erk2) are activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1]) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). MEK1/2 Inhibitor (U0126) inhibits activation of MEK1 and MEK2 with IC50 values of 4uM and 50uM, respectively. SAPK and p38 MAPK are activated by inflammatory cytokines and a wide variety of cellular stresses. Activation of SAPK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* resulting in p38 activation. Phospho-MAPK Family Antibody Sampler Kit contains 40ul of each primary antibody [Phospho-p44/42 MAPK (Thr202/Tyr204), Phospho-SAPK/JNK (Thr183/Tyr185), Phospho-p38 MAPK (Thr180/Tyr182)], 50ul of anti-rabbit IgG secondary antibody (HRP conjugated) and 0.3mg of MEK1/2 Inhibitor (U0126). Applications: Suitable for use in ELISA, Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western IP IHC ICC ELISA Phospho-p44/42 MAPK (Thr202/Tyr204) Ab 1:1000 1:100 1:100 1:250 Phospho-SAPK/JNK (Thr183/Tyr185) Ab 1:1000 NT 1:50 1:2000 Phospho-p38 MAP Kinase (Thr180/Tyr182) Ab 1:1000 1:50 1:100 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: M2352-41
Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 3x40ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Purity: Purified by protein A and peptide affinity chromatography.
References: (1) Favata, M.F. et al. (1998) J. Biol. Chem. 273, 18623
Additional info: Recognizes Phospho-p44/42 MAPK, Phospho-SAPK/ JNK and Phospho-p38 MAPK Antibodies only recognize the phosphorylated form of p44/42 MAPK, SAPK/JNK and p38 MAPK, respectively. They do not appreciably crossreact with other MAPK family members.

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