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MAP Kinase Immunoprecipitation Kinase Assay Kit, BioAssay(TM)

MAP Kinase Immunoprecipitation Kinase Assay Kit, BioAssay(TM)

Cat no: M2353-50

Supplier: United States Biological
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The assay kit is designed to measure phosphotransferase activity in an immunocomplex formed between the MAP Kinase R2 antibody and MAP Kinase (p44mpk). This precipitated enzyme is used to phosphorylate a specific substrate, myelin basic protein (MBP). The phosphorylated substrate is then analyzed by western immunoblot using an antibody specific for phosphorylated MBP. The measurement of MAPK activity in most cell lysates is not accurate, due to the phosphorylation of MBP by other kinases. This enzyme assay is rapid, convenient and specific for MAP Kinase Applications: Non-radioactive immunoprecipitation and measurment of MAP kinases Capacity: 20 assays Components: 5X ADB, (20-145) Anti-MAP Kinase 1/2 agarose Anti-Phospho-MBP MAPK Substrate Cocktail MAPK Inhibitor Cocktai Mg/ATP Cocktail Other components required but not included as part of kit are:
Catalogue number: M2353-50
Applications: Immunoprecipitation, Western Blot
Size: 1Kit
References: 1. Yon, M., et al., J. Neuroimmuno. 58: 121-129, 1995. 2. Yon, M., et al., J. Neuroimmuno. 65: 55-59, 1996. General Kit Reference: Alessi, D.R., et al., Methods Enzymol. 255: 279-289, 1995.

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