MCP-1, also known as CCL2, is expressed by a range of cells including monocytes, vascular endothelial cells, smooth muscle cells, osteoblasts and glomeruler mesangial cells. MCP-1 plays an important role in the inflammatory response of blood monocytes and tissue macrophages.
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Western Blot: 1:1000
Immunohistochemistry (Frozen or paraffin sections): 1:250-1:500
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.