M-CSF R (macrophage colony stimulating factor receptor, c-fms) is a 165kD, 977 amino acid (aa) type I membrane gylcoprotein. It is a member of the type III subfamily of receptor tyrosine kinases that contain five extracellular immunoglobulin-like domains and a cytosolic split kinase
domain. M-CSF receptor is expressed primarily on monocytes, macrophages, dendritic cells, and stem cells, and regulates lineage decisions and maturation of these cells. The mouse M-CSF R extracellular domain shares > 99% aa identity with rat and 60-63% aa identity with human, dog, cat and bovine M-CSF R. It can be released by TACE/ADAM17 cleavage.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: Designed to quantitatively determine the percentage of cells bearing M-CSF R within a population and qualitatively determine the density of M-CSF R on cell surfaces by flow
cytometry. Cell surface expression of M-CSF R is determined by flow cytometry using 488nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 565-605nm. J774 cells were stained with PE-conjugated anti-mouse M-CSF R or isotype control.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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