LYVE-1, also named extracellular link domain containing 1, is a 60-70kD type I transmembrane protein that functions as a receptor for both soluble and immobilized hyaluronan (HA). It shares 41% aa sequence homology with CD44, another known receptor for HA. LYVE-1 expression is mostly restricted to lymphatic vessels. LYVE-1 is a useful molecular marker for lymphatic endothelial cells.
Suitable for use in direct ELISA and Blocking of receptor-ligand interaction. Other applications not tested.
Blocking of receptor-ligand interaction: 0.04-0.2ug/ml
Optimal dilutions to be determined by the researcher.
Endotoxin: (same/less than)0.1EU/ug (LAL)
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.