The low density lipoprotein receptor-related protein-associated protein 1 (LRPAP1/RAP) is an antagonist and molecular chaperone protein that binds tightly to low-density lipoprotein receptor family members and thereby prevents the binding of all known ligands, including alpha 2-macroglobulin and chylomicron remnants. RAP is present on the cell surface where it is an effective inhibitor of ligand binding to the kidney glycoprotein 330 (gp330), but is most abundant in the endoplasmic reticulum lumen and may function in receptor folding and/or trafficking.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.