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Latency Associated Peptide, Transforming Growth Factor beta1 (LAP, TGFb1) (PE)

Cat no: L1393-30

Supplier: United States Biological
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TGF-beta is secreted by cells in the form of an inactive complex. This complex consists of TGF-beta associated non-covalently with a protein designated the latency associated peptide (LAP). TGF-beta and LAP represent components of a pro-peptide that is cleaved in a post-golgi compartment prior to secretion. LAP and TGF-beta each consist of a disulfide-linked homodimer and the association of these two components renders TGF-beta inactive and inaccessible to anti-TGF-beta antibodies. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels (same/less than)1x10e6 cells. Validated using CD4+CD25+ human PBMCs and mouse splenocytes. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human or mouse IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking add 10ul of L1393-30 to (same/less than)1x10e6 cells and incubate for 30 minutes at RT. 3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (Catalog # F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human or Mouse Lyse Buffer. 4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for analysis, cells in a separate tube should be treated with PE-labeled mouse IgG1 antibody. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Stable for at least 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Catalogue number: L1393-30
Reactivities: Human, Mouse
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a liquid in PBS, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Border, W.A. and E. Ruoslahti, 1992, J. Clin. Invest. 90:1-7. 2. Harpel, J.G. et al., 1992, Prog. Growth Factor Res. 4:321-35.
Additional info: Recognizes human LAP (TGFb1). Species Crossreactivity: Mouse

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