Expression of LAMP1/CD107a is determined by flow cytometry using 620-650 nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 660-670 nm.
Suitable for Flow Cytometry. Other Applications not tested.
Optimal dilutions to be determined by the researcher.
Hybridoma: Mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human LAMP-1 (rhLAMP1; aa 28-380).
Storage and Stability:
May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing APC conjugates will result in a substantial loss of activity. APC conjugates are sensitive to light.