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Lactobacilli MRS Broth, Defined, w/o Thymine (Powder)

Cat no: L1021-04

Supplier: United States Biological
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This is a special chemically-defined medium without Thymine or Thymidine. Suitable for use in the cultivation of Lactobacilli from dairy products and other materials. Lactobacilli MRS Broth is based on the formulations of deMan, Rogosa and Sharpe (MRS). This medium supports luxuriant growth of Lactobacilli from oral, fecal, dairy and other sources. Animal tissue digest, beef extract and yeast extract, normally the carbon, nitrogen and vitamin sources used to satisfy general growth requirements in Lactobacilli MRS Broth, are absent and replaced with supplements and vitamins. Dextrose is the fermentable carbohydrate. Potassium acetate and ammonium citrate act as selective agents as well as energy sources. Potassium phosphate is the buffering agent. Magnesium sulfate and manganese sulfate provide cations used in metabolism. Polysorbate (Tween) 80 is a surfacant, facilitating uptake of nutrients by Lactobacilli.
Catalogue number: L1021-04
Size: 10L
Alternative names: LBS Broth
References: 1. J. Applied Micro., 88:695-703 (2000) 2. deMan, J. C., M. Rogosa and M. E. Sharpe, 1960. A medium for the cultivation of lactobacilli. J. Bacteriol. 23:130. 3. MacFaddin, J. F. 1985. Media for the isolation, cultivation, identification and maintenance of medical bacteria, vol. 1 Williams & Wilkins, Baltimore, MD. 4. Vanderzant, C. and D. F. Splittstoesser (eds.). Compendium of methods for the microbiological examination of foods, 3rd ed. American Public Health Association, Washington, D. C. 5. Murray, P. R., E. J. Baron, M. A. Pfaller, F. C. Tenover and R. H. Yolken (eds.). 1995. Manual of clinical microbiology, 6th ed. American Society for Microbiology, Washington, D. C.f

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