KPNA2 is a 58-60kD member of the Importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. KPNA2 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. For the MRN DNA repair complex, KPNA2 first binds to NBS1, and then to Importin b1, which mediates movement through the nuclear pore. Human KPNA2 is 529aa in length. It contains an N-terminal IBB/Importin b domain aa2-60, ten Armadillo repeats that bind "cargo" aa71-496 and two intervening NLS binding sites. There is one splice variant that shows a deletion of aa72-310, coupled to an 11aa substitution for aa389-406. Over aa1-132, human KPNA2 shares 94aa identity with mouse KPNA2.
Applications:
Suitable for use in Western Blot and immunoprecipitation. Other applications not tested.
Recommended Dilution:
Western Blot: 1:500-1:2000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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