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Kappa Chain (B Cells) (FITC)

Cat no: K0080-06A


Supplier: United States Biological
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Human immunoglobulins are glycoproteins composed of two disulfide-bonded heavy (H) chain subunits, each of which is linked by interchain disulfide bonds to a light (L) chain forming a tetramolecular complex. There are five classes of immunoglobulins, designated IgG, IgA, IgM, IgD and IgE, which are defined by differences in the constant region of H chains. L chains are divided into kappa or lambda classifications based on structural antigenic differences. All classes of immunoglobulins have been found on the cell surface of B lymphocytes where they function as antigen receptors to elicit antigen-dependent proliferation and secretion of antigen specific soluble circulating antibodies. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: K0080-06A
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 5% glycerol, 0.2% BSA, 0.04% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. Basic and Clinical Immunology, Seventh edition (D. P. Sites & A. I. Terr, eds.) Appleton & Lange., Norwalk, CT (1991).
Additional info: Recognizes cell surface kappa on B cells.

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