Home  >  Products  >  JNK1/2, phosphorylated (Thr183, Tyr185) (c-Jun N-terminal Kinase 1, JNK1, JNK, c-Jun N-terminal Kinase 2, JNK2, JNK1A2, JNK21B1/2, JNK-46, JNK-55, Mitogen-activated Protein Kinase 8, MAP Kinase 8, MAPK 8, MAPK8, PRKM8, Mitogen-activated Protein Kinase 9,

JNK1/2, phosphorylated (Thr183, Tyr185) (c-Jun N-terminal Kinase 1, JNK1, JNK, c-Jun N-terminal Kinase 2, JNK2, JNK1A2, JNK21B1/2, JNK-46, JNK-55, Mitogen-activated Protein Kinase 8, MAP Kinase 8, MAPK 8, MAPK8, PRKM8, Mitogen-activated Protein Kinase 9,

Cat no: J2500-10E


Supplier: United States Biological
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The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and g radiation, ceramides, inflammatory cytokines and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4-7, which then phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4-7 can be activated by a pathway independent of small GTPases via stimulation of a member of the germinal center kinase (GCK) family (4). There are three SAPK/JNK genes with further diversification resulting from alternative splicing (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus where it regulates transcription through its effects on c-Jun, ATF-2 and other transcription factors (3,5). Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:50. Requires citrate unmasking buffer. Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: J2500-10E
Reactivities: Human, Mouse, Rat, Drosophila/Arthropod, Yeast/Fungi
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
Alternative names: EC=2.7.11.24
References: 1. Davis, R.J. (1999) Biochem. Soc. Symp. 64, 1-12. 2. Ichijo, H. (1999) Oncogene 18, 6087-6093. 3. Kyriakis, J.M. and Avruch, J. (2001) Physiol. Rev. 81, 807-869. 4. Kyriakis, J.M. (1999) J. Biol. Chem. 274, 5259-5262. 5. Leppa, S. and Bohmann, D. (1999) Oncogene 18, 6158-6162. 6. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.
Additional info: Recognizes endogenous levels of human p46 and p54 SAPK/JNK only when phosphorylated at Thr183 and Tyr185. Does not recognize phosphorylated p44/42 or p38 MAP kinases. Species Crossreactivity: mouse, rat, D.melanogaster and S.cerevisiae.

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