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Isocitrate Dehydrogenase, Recombinant (IDH1)

Cat no: I8850-01

Supplier: United States Biological
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Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle. Recombinant MDH is purified by proprietary chromatographic techniques. Unit Definition: One unit is defined as 1umol of NAD+ production per minute under the assay conditions (25 degrees C, pH 7.5). Storage and Stability: For long-term storage, add HSA or BSA (0.1% final concentration), aliquot and store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: I8850-01
Size: 1mg
Form: Supplied as a liquid in potassium phosphate, 50% glycerol, pH 7.1.
Purity: (same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
References: 1. Expression of cytosolic NADP+-dependent isocitrate dehydrogenase in bovine mammary epithelium: Modulation by regulators of differentiation and metabolic effectors. Exp Biol Med (Maywood) 2006 May;231(5):599-610 2. Crystallization and preliminary X-ray crystallographic analysis of yeast NAD+-specific isocitrate dehydrogenase. Acta Crystallograph Sect F Struct Biol Cryst Commun 2005 May 1;61(Pt 5):486-8 3. Substrate-free structure of a monomeric NADP isocitrate dehydrogenase: an open conformation phylogenetic relationship of isocitrate dehydrogenase. Proteins 2006 Apr 1;63(1):100-12 4. Control of isocitrate dehydrogenase catalytic activity by protein phosphorylation in Escherichia coli. J Mol Microbiol Biotechnol 2005;9(3-4):132-46 5. The RpoS-mediated regulation of isocitrate dehydrogenase gene expression in Escherichia coli. Curr Microbiol 2006 Jan;52(1):21-6 6. Analysis of interactions with mitochondrial mRNA using mutant forms of yeast NAD(+)-specific isocitrate dehydrogenase. Biochemistry 2005 Dec 20;44(50):16776-84

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