CXCL8, also known as IL-8, is a chemoattractant that recruits neutrophils to sites of inflammation, activates neutrophil functions and promotes angiogenesis. CXCL8 production is rapidly induced by pro-inflammatory signals in many different cell types. The biological activites of CXCL8 are mediated by chemokine receptors CXCR1 and CXCR2. Feline CXCL8 is translated as a 101aa precursor protein with a 22aa signal peptide. It shares 61% and 76% aa sequence identity with human and canine CXCL8, respectively.
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
ELISA: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.