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Interleukin 6 Receptor (CD126, IL-6R) (Carboxyfluorescein)

Cat no: I8428-39L2

Supplier: United States Biological
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The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80kD component receptor that binds IL-6 with low affinity (IL-6 R) and a signal-transducing component of 130kD (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R and gp130 have been cloned, sequenced, and expressed . A soluble form of the IL-6 R has been found in the urine of healthy adult humans. This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R. No naturally-occurring mRNA encoding a truncated form of the IL-6 R has been reported. Soluble forms of human and murine IL-6 Rs have been constructed, however, by insertion of termination codons into the regions of the IL-6 R cDNAs encoding the external portions of the receptors prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R complex to membrane-bound gp130. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Use 10ul to label 10e6 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: I8428-39L2
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with Carboxyfluorescein.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes human IL-6R.

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