The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80kD component receptor that binds IL-6 with low affinity (IL-6 R) and a signal- transducing component of 130kD (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R and gp130 have been cloned, sequenced, and expressed.
A soluble form of the IL-6 R has been found in the urine of healthy adult humans . This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R. No naturally-occurring mRNA encoding a truncated form of the IL-6 R has been reported. Soluble forms of human and murine IL-6 Rs have been constructed, however, by insertion of termination codons into the regions of the IL-6 R cDNAs encoding the external portions of the receptors prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R complex to membrane-bound gp130.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: Neat. Use 10ul to label 1x10e5 cells.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing APC conjugates will result in a substantial loss of enzymatic activity. APC conjugates are sensitive to light.
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