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Interleukin 5 (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF) (Carboxyfluorescein)

Cat no: I8427-03F

Supplier: United States Biological
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The presence or absence of distinct cytokines determines the quality and quantity of the immune response following antigenic challenge (1, 2). Immune responses range from a cellular (macrophage/cytotoxic T cell) response (3-5), to a predominantly humoral or antibody-mediated immunity (6). IL-2, IL-12, IFN-y and TNF-b are associated with cellular immunity (7-9), while IL-4, -5, -6, and -10 tend to accompany antibody responses (10-12). IL-2, IL-12, IFN-y and TNF-b are known as type 1 cytokines. IL-4, -5, -6, -10 and -13 are referred to as type 2 cytokines (13). T helper cells (Th1 and Th2) are considered end products of CD4+ T cell differentiation. Th1 cells secrete IL-2, TNF-b and IFN-y. Th2 cells generate IL-4, -5, -6, and -10. CD4+ precursors are referred to as Thp or Th0 cells, since they secrete a combination of both type 1 and type 2 cytokines (14). Although this original Th1/Th2 model has been a tool for defining complex T cell interactions, it is now apparent that some modifications may be required. For example, Th1 and Th2 cytokines are not mutually exclusive, they can occur in many combinations (IL-5 with IFN-g being a notable exception) (14-16). In addition, CD4+ T cells are not the exclusive source of cytokine(s) for various effector cells, CD8+ (or cytotoxic) T cells can also perform similar functions (17, 18). Type 1 cytokines can be generated from either Th1/CD4+ or Tc1/CD8+ T cells. Type 2 cytokine production is associated with either Th2/CD4+ or Tc2/CD8+ T cells (18). The ability to investigate cytokine expression at the single cell level, by combining the power of flow cytometry and intracellular cytokine staining reagents, may offer new insights into immunity (14, 15). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Use 10ul to label 10e6 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: I8427-03F
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with Carboxyfluorescein.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Petrov, R. (1992) Immune Response Regulation. in Encyclopedia of Immunology, Roitt, I.M. and P.J. Delves, Ed., Academic Press Ltd. San Diego, CA. 2. Kaufmann, S.H.E. (1995) Immunol. Today 16:338. 3. Fitch, F.W. (1992) Cell-Mediated Immunity. in Encyclopedia of Immunology, Roitt, I.M. and P.J. Delves, Ed., Academic Press Ltd. San Diego, CA. 4. Celada, A. and C. Nathan (1994) Immunol. Today 15:100. 5. Apasov, S. et al. (1993) Curr. Opin. Immunol. 5:404. 6. Berek, C. (1992) Humoral Immunity. in Encyclopedia of Immunology, Roitt, I.M. and P.J. Delves, Ed., Academic Press Ltd. San Diego, CA. 7. Waldmann, T. (1993) Immunol. Today 14:264. 8. Trinchieri, G. (1995) Annu. Rev. Immunol. 13:251. 9. Farrar, M.A. and R.D. Schreiber (1993) Annu. Rev. Immunol. 11:571. 10. Spits, H. (1992) IL-4: Structure and function. CRC Press, Inc., Boca Raton, FL. 11. Takatsu, K. et al. (1994) Adv. Immunol. 57:145. 12. Moore, K.W. et al. (1993) Annu. Rev. Immunol. 11:165. 13. Mosmann, T.R. and R.L. Coffman (1989) Annu. Rev. Immunol. 7:145. 14. Kelso, A. (1995) Immunol. Today 16:374. 15. Prussin, C. and D.D. Metcalfe (1995) J. Immunol. Methods 188:117. 16. Elson, L.H. et al. (1995) J. Immunol. 154:4294. 17. Le Gros, G. and F. Erard (1994) Curr. Opin. Immunol. 6:453. 18. Sad, S. et al. (1995) Immunity 2:271.
Additional info: Recognizes human IL-5.

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