Home  >  Products  >  Interleukin 4, Recombinant, Rabbit (Interleukin-4, IL-4, B-cell Stimulatory Factor 1, BSF-1, Lymphocyte Stimulatory Factor 1, IL4)

Interleukin 4, Recombinant, Rabbit (Interleukin-4, IL-4, B-cell Stimulatory Factor 1, BSF-1, Lymphocyte Stimulatory Factor 1, IL4)

Cat no: I8426-43F

Supplier: United States Biological
0 reviews | Write a Review Pencil
Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, ~13kD-18kD Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four a-helix structure. Rabbit IL-4 is synthesized with a 24aa signal sequence. Mature rabbit IL-4 shares 47%, 56%, 39% and 40% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities. IL-4 exerts its effects through two receptor complexes. The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 Ra and the common G chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 Ra and IL-13 Ra1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by nai?ve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. Source: Recombinant corresponding to aa25-147 from rabbit IL-4, fused to N-terminal Met, expressed in E.coli. Molecular Weight: ~14.2kD Biological Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.The ED50 for this effect is typically 3-15ng/ml. Endotoxin: ~1EU/1ug (LAL) Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: I8426-43F
Size: 25ug
Form: Supplied as a lyophilized powder in PBS, BSA. Reconstitute with PBS, 0.1% human or BSA to 0.1mg/ml.
Purity: ~95% (SDS-PAGE)
References: 1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109. 2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol.17:1. 3. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. 83:5894. 4. Redfield, C. et al. (1991) Biochemistry 30:11029. 5. Perkins, H.D. et al. (2000) Cytokine 12:555. 6. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141. 7. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol.111:451. 8. Mosman, T.R. et al. (1987) J. Immunol. 138:1813. 9. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta1592:237. 10. Nelms, K. et al. (1999) Annu.Immunol17:701.11. Paludan, S.R. (1998) Scand. J. Immunol. 48:459. 12. Corthay, A. (2006) Scand. J. Immunol. 64:93. 13. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15. 14. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1. 15. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol.119:1303.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*

Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave