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Interleukin 4 (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin, HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1, Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MGC79402, Pitrakinra, T Cell Grow

Cat no: I8426-32B


Supplier: United States Biological
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Applications: ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-4 protein levels.1-4 Purified antibody should be paired with biotinylated antibody (Cat. No. I8426-04B) as the detection antibody, with purified recombinant mouse IL-4 as the standard. Immunofluorescence/Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant mouse IL-4, unlabeled Rat IgG2b should be used as a specificity control. Western Blot: Useful for Western blot applications. For Western blotting, a concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of (same/less than) 100ng/lane of recombinant mouse IL-4 under reducing conditions. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: I8426-32B
Reactivities: Mouse
Hosts: Rat
Applications: ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Size: 500ug
Form: Supplied as a liquid in 100mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
P type: Mab
Isotype: IgG2b,k
Purity: Purified
References: 1. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24. 2. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and sera. J. Immunol. Meth. 166:201-214. 3. Finkelman, F., K. Madden, S. Morris, J. Holmes, N. Bolani,, I. Jkatona, and C. Maliszewski. 1993. Anti-cytokine antibodies as carrier proteins. J. Immunol. 151:1235-1244. 4. Litton, M., B. Sander, E. Murphy, A. O
Additional info: Recognizes mouse Interleukin-4 (IL-4).

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