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Interleukin 4 (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin, HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1, Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MGC79402, Pitrakinra, T Cell Grow

Cat no: I8426-23A1


Supplier: United States Biological
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Applications: ELISA Detection: Monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human IL-4 protein levels.1,3 Biotinylated antibody should be paired with a suitable purified antibody as the capture antibody, with purified recombinant human IL-4 as the standard. Immunofluorescence/Flow Cytometry: This antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant human IL-4, unlabeled antibody, or Biotinylated Rat IgG1 should be used as a specificity control. Western Blotting: The purified antibody is also useful for Western blotting applications. A concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of (same/less than) 100ng/lane of recombinant human IL-4 under reducing conditions. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: I8426-23A1
Reactivities: Human
Hosts: Rat
Applications: ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Conjugates: Biotin
Size: 500ug
Form: Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.
P type: Mab
Isotype: IgG1,k
Purity: Purified
References: 1. Chretien, I., A.V. Kimmenade, M.K. Pearce, J. Banchereau, and J.S. Abrams. 1989. J. Immunol. Meth. 117:67. 2. Ramanathan, L., R. Ingram, L. Sullivan, R. Greenberg, R. Reim, P. Trotta, and H. Lee. 1993. Biochem. 32:3549. 3. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5.
Additional info: Recognizes human Interleukin-4 (IL-4). Has been reported to crossreact with IL-4 from Rhesus monkeys. Has also been reported to be useful for epitope mapping of human IL-4.

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