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Interleukin 4 (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin, HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1, Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MGC79402, Pitrakinra, T Cell Grow

Cat no: I8426-23


Supplier: United States Biological
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Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence/Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant human IL-4, unlabeled antibody, or Biotinylated Rat IgG1 should be used as a specificity control. ELISA: Useful as a detection antibody in a sandwich ELISA for quantifying human IL-4 protein levels. Western Blot: 1-5ug/ml (in conjunction with AP-labeled Goat anti-Rat-Ig) has been found to enable detection of (same/less than) 100ng/lane of recombinant human IL-4 under reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: I8426-23
Reactivities: Human, Non-Human Primate
Hosts: Rat
Applications: ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Size: 500ug
Form: Supplied as a liquid in 100mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
P type: Mab
Isotype: IgG1,k
Purity: Purified immunoglobulin
References: 1. Chretien, I., et al., J. Immunol. Meth. 117: 67 (1989). 2. Ramanathan, L., et al., Biochem. 32: 3549 (1993). 3. Abrams, J.S., et al., Immunol. Rev. 127: 5 (1992).
Additional info: Recognizes human Interleukin-4 (IL-4). Has been reported to be useful for epitope mapping of human IL-4. Species Crossreactivity: Rhesus monkey.

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