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Interleukin 23R (Interleukin 23 Receptor, IL-23R) (PE)

Cat no: I8443-49A


Supplier: United States Biological
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IL-23R is an IL-23-specific binding protein that associates with IL-12 Rb1 to form a functional heterodimeric receptor complex. This complex mediates biological activities that are similar to, but distinct from, those induced by IL-12. IL-23R is expressed by Th1 and NK cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat. 10ul labels 1x10e5 cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cells should be Fc-blocked by treatment with 1ug of human IgG/10e5 cells for 15 minutes at RT prior to staining. Do not wash excess blocking IgG from this reaction. 2. Transfer 25ul of the Fc-blocked cells (1x10e5 cells) or 50ul of packed whole blood to a 5ml tube. 3. Add 10ul of I8443-49A. 4. Incubate for 30-45 minutes at 4 degrees C. 5. Following this incubation, remove unreacted I8443-49A reagent by washing the cells twice in 4ml of the same PBS buffer (Note: Whole blood will require an RBC lysis step at this point using any commercially available lysing reagent). 6. Finally, resuspend the cells in 200-400ul of PBS buffer for final flow cytometric analysis. 7. As a control for analysis, cells in a separate tube should be treated with PE-labeled mouse IgG2b antibody. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C. Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Catalogue number: I8443-49A
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a liquid in PBS, (same/less than) 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes human Interleukin 23R. Does not crossreact with recombinant mouse IL-23R.

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