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Interleukin 2 (IL-2) (PE)

Cat no: I7663-29D

Supplier: United States Biological
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The cytokine human interleukin-2 (IL-2) is a 15kD protein expressed upon activation by peripheral TH1 cells, medullary thymocytes, and a subset of large granular lymphocytes. Secreted IL-2 stimulates proliferation and lymphokine production by T cells, B cells and NK cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50. 80ul labels 5x10e5 cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Ficoll prepared human peripheral blood lymphocytes were stimulated 4 hours in the presence of 50ng/ml PMA, 2uM ionomicin and 5uM monensin. 2. Cells were then harvested, washed and fixed for 30 min with 2% buffered formaldehyde after which they were washed two times. 3. Fixed cells were permeabolized 10 minutes in a buffer containing 0.3% Saponin. Subsequent reagent incubations and washes were done using this buffer. 4. 5x10e5 cells per tube were preincubated 5 minutes with 20ul of 0.25mg/ml human IgG (To block nonspecific binding), after which they were incubated 45 minutes on ice with 80ul of I7663-29D at a 1:50 dilution (5ug/ml). 5. Cells were washed three times, fixed and analyzed by FACS. 6. A net 26% sub population of the cells stained positive with a mean shift of 0.94 log10 fluorescent units when compared to a Mouse IgG2a/R-PE negative control (I1904-84B2) at a similar concentration. 7. Binding was blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml anti-IL-2 antibody Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Catalogue number: I7663-29D
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
P type: Mab
Isotype: IgG2a,k
Purity: Purified by Protein A affinity chromatography.
References: 1.S.L. Swain (1991) Curr Opin Immunol 3-304-310. 2. A.K. Abbas, et al, (1996) Nature 383: 787-793. 3. A. Rebollo, et al, (1996) Advan Immunol 63: 127-196.
Additional info: Recognizes human Interleukin 2.

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