Interleukin-17F (also known as ML-1) is a newly discovered member of the IL-17 family of cytokines. Members of this family are involved in tissue homeostasis and demonstrate a structural motif termed a cysteine knot that characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intra-chain disulfide bonds to create the knot, IL-17 family molecules adapt the same structural form with only two disulfide links.1-3 Human IL-17F is secreted as a glycosylated, 45kD, disulfide-linked homodimer. It is synthesized as a 153 amino acid (aa) precursor that contains a 20 aa signal sequence and a 133 aa mature region. As noted, the molecule contains a cysteine knot that is formed from two sets of paired Beta-strands that are connected by disulfide linkages.4-6 There is potentially one additional isoform that utilizes an alternate start site. This adds an additional 10 amino acids at the N-terminus.1, 7 Its significance is unknown. When induced, IL-17F is preferentially translated as the 153 aa precursor.1 Initially, IL-17F was also reported as IL-24.7 Since that time, the IL-24 designation has been reassigned to MDA-7, a member of the IL-10 family of molecules (note: IL-17E is synonymous with IL-25). Mature human IL-17F is 59%, 56%, 44% and 52% identical to mature rat, mouse, canine and bovine IL-17F, respectively. Within the IL-17 family, IL-17F is most closely related to IL-17/IL-17A at 50% aa identity.6 IL-17F is produced by activated CD4+ T cells, mast cells, basophils and monocytes,1, 4 and can be induced by IL-23.4, 8 Targets for IL-17F include respiratory epithelium and endothelial cells which produce pro-inflammatory cytokines that promote neutrophil activation and influx. (4, 5, 9, 10)
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: Use 10ul to label 10e6 cells.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.