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Interleukin 17F (IL-17F) (Carboxyfluorescein)

Cat no: I8439-28F


Supplier: United States Biological
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Interleukin-17F (also known as ML-1) is a newly discovered member of the IL-17 family of cytokines. Members of this family are involved in tissue homeostasis and demonstrate a structural motif termed a cysteine knot that characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intra-chain disulfide bonds to create the knot, IL-17 family molecules adapt the same structural form with only two disulfide links.1-3 Human IL-17F is secreted as a glycosylated, 45kD, disulfide-linked homodimer. It is synthesized as a 153 amino acid (aa) precursor that contains a 20 aa signal sequence and a 133 aa mature region. As noted, the molecule contains a cysteine knot that is formed from two sets of paired Beta-strands that are connected by disulfide linkages.4-6 There is potentially one additional isoform that utilizes an alternate start site. This adds an additional 10 amino acids at the N-terminus.1, 7 Its significance is unknown. When induced, IL-17F is preferentially translated as the 153 aa precursor.1 Initially, IL-17F was also reported as IL-24.7 Since that time, the IL-24 designation has been reassigned to MDA-7, a member of the IL-10 family of molecules (note: IL-17E is synonymous with IL-25). Mature human IL-17F is 59%, 56%, 44% and 52% identical to mature rat, mouse, canine and bovine IL-17F, respectively. Within the IL-17 family, IL-17F is most closely related to IL-17/IL-17A at 50% aa identity.6 IL-17F is produced by activated CD4+ T cells, mast cells, basophils and monocytes,1, 4 and can be induced by IL-23.4, 8 Targets for IL-17F include respiratory epithelium and endothelial cells which produce pro-inflammatory cytokines that promote neutrophil activation and influx. (4, 5, 9, 10) Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Use 10ul to label 10e6 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: I8439-28F
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. Labeled with Carboxyfluorescein.
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein G affinity chromatography.
Alternative names: ML-1
References: 1. Kawaguchi, M. et al., 2004, J. Allergy Clin. Immunol. 114:1265.2. Kolls, J.K. and A. Linden, 2004, Immunity 21:467.3. Moseley, T.A. et al., 2003, Cytokine Growth Factor Rev. 14:155.4. Kawaguchi, M. et al., 2001, J. Immunol. 167:4430.5. Starnes, T. et al., 2001, J. Immunol. 167:4137.6. Hymowitz, S.G. et al., 2001, EMBO J. 20:5332.7. Hromes, R.A. and T.T. Starnes, 2001, GenBank Accession # AAK83350.8. Langrish, C.L. et al., 2005, J. Exp. Med. 201:233.9. Mcallister, F. et al., 2005, J. Immunol. 175:404.10. Numasaki, M. et al., 2004, Immunol. Lett. 95:175.
Additional info: Recognizes human IL-17F.

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