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Interleukin 17F (IL-17F) (APC)

Cat no: I8439-28E

Supplier: United States Biological
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Interleukin-17F (also known as ML-1) is a newly discovered member of the IL-17 family of cytokines. Members of this family are involved in tissue homeostasis and demonstrate a structural motif termed a cysteine knot that characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intra-chain disulfide bonds to create the knot, IL-17 family molecules generate the same structural form with only two disulfide links. Human IL-17F is secreted as a glycosylated, 45kD, disulfide-linked homodimer. It is synthesized as a 153 aa precursor that contains a 20 aa signal sequence and a 133 aa mature region. As noted, the molecule contains a cysteine knot that is formed from two sets of paired beta-strands that are connected by disulfide linkages. There is potentially one additional isoform that utilizes an alternate start site. This adds an additional 10 amino acids at the N-terminus.1, Its significance is unknown. When induced, IL-17F is preferentially translated as the 153 aa precursor.1 Initially, IL-17F was also reported as IL-24.7 Since that time, the IL-24 designation has been reassigned to MDA-7, a member of the IL-10 family of molecules (note: IL-17E is synonymous with IL-25). Mature human IL-17F is 59%, 56%, 44% and 52% identical to mature rat, mouse, canine and bovine IL-17F, respectively. Within the IL-17 family, IL-17F is most closely related to IL-17/IL-17A at 50% aa identity. Human IL-17F is active on mouse and pig cells.6 IL-17F is produced by activated CD4+ T cells, mast cells, basophils and monocytes1, 4 and is inducible by IL-23. Targets for IL-17F include respiratory epithelium and endothelial cells which produce pro-inflammatory cytokines that promote neutrophil activation and influx. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 5x10e5 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: I8439-28E
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a liquid in PBS, pH 7.4, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein G affinity chromatography.
Alternative names: ML-1
References: 1. Kawaguchi, M. et al., 2004, J. Allergy Clin. Immunol. 114:1265. 2. Kolls, J.K. and A. Linden, 2004, Immunity 21:467, 3. Moseley, T.A. et al., 2003, Cytokine Growth Factor Rev. 14:155. 4. Kawaguchi, M. et al., 2001, J. Immunol. 167:4430. 5. Starnes, T. et al., 2001, J. Immunol. 167:4137. 6. Hymowitz, S.G. et al., 2001, EMBO J. 20:5332. 7. Hromes, R.A. and T.T. Starnes, 2001, GenBank Accession # AAK83350. 8. Langrish, C.L. et al., 2005, J. Exp. Med. 201:233. 9. McAllister, F. et al., 2005, J. Immunol. 175:404. 10. Numasaki, M. et al., 2004, Immunol. Lett. 95:175.
Additional info: Recognizes human IL-17F.

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