The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions. Mouse IL-17E cDNA encodes a 169 amino acid (aa) precursor protein with a putative 16 aa signal peptide. Mouse IL-17E shares from 24% to 32% sequence identity with the other mouse IL-17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland, spinal cord, and trachea. IL-17E binds and activates the IL-17 B Receptor (IL-17B R; alternatively known as IL-17 Rh1, IL-17E R, and EVI27) which is expressed in the kidney and liver, and at lower levels in the brain, testis and other endocrine tissues. The expression of IL-17B R is up-regulated under inflammatory conditions. Ligation of IL-17E to IL-17B R induces activation of nuclear factor kappa-B and stimulates the production of the proinflamatory cytokine IL-8. IL-17 has also been found to promote the expression of the prototypical Th2 genes.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: Neat. Use 10ul to label 5x10e5 cells.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.