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Interleukin 12 Receptor, beta 2 (IL-12Rb2) (PE)

Cat no: I8434-50B

Supplier: United States Biological
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The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of the IL-12 receptor b1 (IL-12 Rb1) and IL-12 receptor b2 (IL-12 Rb2) subunits. IL-12 Rb2 is a 130kD protein that interacts with p35 and is associated with Jak2. The expression of IL-12 Rb2 is absent on nai?ve T cells. Activation of T cells via TCR upregulates IL-12 Rb2 expression on human Th1, but not Th2 cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells. Validated using human Th1-differentiated PBMCs. Human CD4+ peripheral blood mononuclear cells (PBMCs) treated with mouse anti-human CD3e monoclonal antibody, Recombinant human IL-12 and Recombinant human IL-2 to induce Thi cell development, followed by restimulation with PMA and calcium Ionomycin. Optimal dilutions to be determined by the researcher. Hybridoma: NSO myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Catalogue number: I8434-50B
Reactivities: Human, Mouse
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a liquid in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Trinchieri, G. et al. (2003) Immunity 19:641. 2. Brombacher, F. et al. (2003) Trends Immunol. 23:207. 3. Trinchieri, G. (2003) Nat. Rev. Immunol. 3:133. 4. Rogge, L. et al. (1997) J. Exp. Med. 185:825.
Additional info: Recognizes human Interleukin 12 Receptor, beta 2 (IL-12Rb2). Species Crossreactivity: mouse. In direct ELISA, no reactivity with recombinant human IL-1 R beta 1 is observed.

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