The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of the IL-12 receptor b1 (IL-12 Rb1) and IL-12 receptor b2 (IL-12 Rb2) subunits. IL-12 Rb2 is a 130kD protein that interacts with p35 and is associated with Jak2. The expression of IL-12 Rb2 is absent on nai?ve T cells. Activation of T cells via TCR upregulates IL-12 Rb2 expression on human Th1, but not Th2 cells.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: 10ul labels 1x10e6 cells. Validated using human Th1-differentiated PBMCs. Human CD4+ peripheral blood mononuclear cells (PBMCs) treated with mouse anti-human CD3e monoclonal antibody, Recombinant human IL-12 and Recombinant human IL-2 to induce Thi cell development, followed by restimulation with PMA and calcium Ionomycin.
Optimal dilutions to be determined by the researcher.
NSO myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.