IFN gamma is a regulatory cytokine, which is produced by activated natural killer cells and a population of T-lymphocytes.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: Neat, Use 10ul to label 10e6 cells in 100ul. Membrane permeabilisation is required for this application.
Optimal dilution determined by the researcher
Recommended Negative Controls:
Mouse IgG1
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.