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Interferon, gamma (PE)

Cat no: I7662-15H1

Supplier: United States Biological
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IFN gamma is a regulatory cytokine, which is produced by activated natural killer cells and a population of T-lymphocytes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat, Use 10ul to label 10e6 cells in 100ul. Membrane permeabilisation is required for this application. Optimal dilution determined by the researcher Recommended Negative Controls: Mouse IgG1 Storage and Stability: Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: I7662-15H1
Reactivities: Human, Non-Human Primate
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a lyphilized powder in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Conjugated to R. Phycoerythrin (RPE).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Favre. C. et al. (1989). Epitope mapping of recombinant human gamma interferon using monoclonal antibodies. Mol. Immunol. 26:17-25 .2. Abrams. J. et al. (1992). Strategies of anti-cytokine monoclonal antibody development. Immunol. Rev. 127:5-24. 3. Palliard. X. et al. (1988). Simultaneous production of IL-2, IL-4, and IFN gamma by activated human CD4+ and CD8+ T cell clones. J. Immunol. 141:849-855. 4. Baig. J. et al. (2002). Elicitation of Simian Immunodeficiency virus-specific cytotoxic T lymphocytes in mucosal compartments of rhesus monkeys by systemic vaccination. J. Virol. 76:11484-11490.
Additional info: Specific for human interferon gamma (IFN gamma). Species Cross Reactivity: Rhesus Monkey.

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