Interferon gamma (IFNg) (PE)

Interferon-g is an important immunoregulatory cytokine that plays a key role in host defense by exerting antiviral, antiproliferative and immunoregulatory activities. Bovine IFN-g is synthesized as 166 amino acid (aa) precursor that contains a 20 aa signal sequence and a 146 aa mature region. Based on human studies, bovine IFN-g exists as an a-helical, noncovalently linked intercalated homodimer. In the mature segment, bovine IFN-g is 61%, 79%, 97%, 45%, 44%, and 55% aa identical to human, porcine, ovine, rat, mouse, and cotton rat IFN-g, respectively. The production of IFN-g is upregulated synergistically by IL-12, IL- 18, IL-23, and IL-27. IFN-g induces cytokine release and promotes the expression of various membrane proteins. This includes class I and II MHC antigens, Fc receptors, and leukocyte adhesion molecules. IFN-g is a potent activator of macrophage effector functions. It also inhibits Th2 differentiation, stimulates Th1 development, and influences mononuclear cell chemotaxis. It influences chemotaxis by inducing the synthesis of CXC chemokines 9 and 10, and CC chemokines 2, 3, 4, and 5. The functional IFN-g receptor complex consists of two distinct subunits. An a subunit (IFN-g R1) binds IFN-g with high affinity, while a b-subunit (IFN-g R2) interacts with the IFN-g occupied a-subunit to transduce a signal. Each IFN-g dimer interacts with two a?b subunit receptor pairs. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.