The cytokine human interferon-gamma (IFN-gamma) is a glycosylated protein that exists as a homodimer of 34 kd in its biologically active form. IFN-gamma is expressed upon activation by TH1 cells CD8+ cells and NK cells (2). Secreted IFN-gamma is a potent inhibitor of in vitro hematopoiesis and appears to serve as an important regulator of lymphocyte and macrophage function by exerting antiproliferative effects (1).
Applications:
Suitable for use in Flow Cytometry (intracellular). Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Store at 2-8 degrees C. Do Not Freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.